GHRH growth hormone releasing hormone Somatoliberin, Growth hormone-releasing factor, GRF, Growth hormone-releasing hormone, GHRH, Somatocrinin, Somatorelin, Sermorelin, GHRF, MGC119781. Growth-hormone-releasing hormone (GHRH), also known as growth-hormone-releasing factor (GRF or GHRF) or somatocrinin, is a 44-amino acidpeptide hormoneproduced in the arcuate nucleusof the hypothalamus. GHRH is released from neurosecretory nerve terminals of these arcuate neurons, and is carried by the hypothalamo-hypophysial portal circulation to the anterior pituitary glandwhere it stimulates growth hormone(GH) secretion. GHRH also stimulates the production of GH. GHRH is released in a pulsatile manner, stimulating similar pulsatile release of GH. In addition, GHRH also promotes slow-wave sleepdirectly. Description Growth Hormone Releasing Hormone Human Synthetic is a single, non-glycosylated, polypeptide chain containing 29 amino acids and having a molecular mass of 3358.9 Dalton. Corresponds to the amino-terminal segment of the naturally occurring human growth hormone-releasing hormone consisting of 44 amino acid residues. The GHRH is purified by proprietary chromatographic techniques. Sterile Filtered White lyophilized (freeze-dried) powder. Formulation The GHRH peptide (1mg/ml) was lyophilized after extensive dialyses against 1.7 mg sodium phosphate buffer (0.1 mg sodium phosphate monobasic & 1.6 mg sodium phosphate dibasic). Solubility It is recommended to reconstitute the lyophilized GHRH in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. The GHRH is also soluble in 1% Acetic acid at a concentration of >1mg/ml to give a clear, colorless solution. Stability Lyophilized Growth Hormone Releasing Hormone although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GHRF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Purity Greater than 98.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. Amino Acid Sequence The free base of sermorelin has the empirical formula C149H246N 44O42S. Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-NH2.